Abstract

Posttranslational modifications, particularly phosphorylation, regulate activity, stability and localization of proteins in circadian clocks, thereby contributing to a stable oscillation with a period of approximately 24 h. The White Collar Complex (WCC) is the central transcription factor of the circadian clock of Neurospora crassa. Its activity is regulated in a circadian manner by rhythmic phosphorylation, mediated by the clock protein Frequency (FRQ). Here we present purification of TAP-tagged WCC and identification of novel phosphorylation sites of WC-1 and WC-2, all of which appear to be proline directed. Exchange of a single WC-2 serine residue (S433) to alanine or aspartate affects WCC-dependent transcription and circadian period, suggesting an important role of WC-2 S433 phosphorylation for WCC activity and circadian timing. Structured summary MINT- 7033869: WC-2 (uniprotkb: P78714) physically interacts (MI: 0218) with WC-1 (uniprotkb: Q01371) by tandem-affinity purification (MI: 0676) MINT- 7033885: WC-2 (uniprotkb: P78714) physically interacts (MI: 0218) with FRQ (uniprotkb: P19970) and WC-1 (uniprotkb: Q01371) by cross-linking studies (MI: 0030)

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