Abstract
The aim of the work is to study the functional characteristics of SLC transporters of organic anions: OAT1 and OAT3 in normal conditions and in model ischemia/reperfusion injury.Materials and methods. The HEK293 cell line was used as a model for the study. Conditions of ischemia/reperfusion injury were created by the previously described method. The activity of the transporters was assessed by the capture of the marker substrate - fluorescein. The concentration of fluorescein was measured using a plate fluorimeter. The results were normalized by the amount of total protein.Results. In condition of ischemia/reperfusion injury, the activity of organic anion transporters decreased in comparison with the norm. The data obtained allow us to conclude that in conditions of ischemia/reperfusion injury, the concentration of dicarboxylic acids in the cell is low, which in turn can lead to a decrease activity of transporters.
Highlights
ВВЕдЕНиЕ Система транспортеров органических анионов (ОАТ) играет важную роль в выведении широкого спектра эндогенных соединений и ксенобиотиков
Fluorescein uptake is expressed as pmol per mg cell protein and the time of incubation (10 min)
The authors declare that there is not conflict of interests
Summary
ВВЕдЕНиЕ Система транспортеров органических анионов (ОАТ) играет важную роль в выведении широкого спектра эндогенных соединений и ксенобиотиков. OAT1 и OAT3 осуществляют захват органических анионов внутрь клеток из крови через базальную мембрану в обмен на анионы дикарбоновых кислот [2]. Повышенная активность ОАТ служит одной из причин накопления ксенобиотиков в клетках проксимальных канальцев и связанной с этим нефротоксичности [3]. Цель исследования – оценить влияние постишемической реперфузии на активность транспортеров органических анионов на модели клеточной линии НЕК29. МАтЕриАлы и МЕтоды Генетитический паспорт клеточной линии HEK293
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