Abstract
The characteristics of recombinant phytase from Obesumbacterium proteus have been studied after its integration into Yarrowia lipolytica with a Po1f (pUV3-Op) plasmid. Phytase activity was observed in Y. lipolytica Po1f transformants in a wide pH range (3.5–7.5) and remained at 80% of the initial value after 3-min heating at 95°C, which made it possible to add the enzyme to a fodder produced with heat treatment. In the case of transformant cultivation on low-grade vegetable substrates, they also possessed confirmed phytase activity, as well as an increase in the content of intracellular phosphates as compared to cells of the wild-type Y. lipolytica strain.
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