Activity of Lactic Acid Bacteria (LAB) from the intestines of vannamei shrimp (Lithopenaeus vannamei) in inhibiting the growth of Vibrio harveyi

Abstract

Lactic Acid Bacteria (LAB) is a bacterium that has been widely used as a probiotic because it can inhibit the growth of pathogenic bacteria. Lactic acid bacteria are usually found in the digestive tract of both humans and animals. In this study, isolates of Lactic Acid Bacteria from the intestines of vannamei shrimp (Litopenaeus vannamei) were isolated from traditional ponds in Bangkalan. Antibacterial activity testing was carried out on Vibrio harveyi, and then the suspected isolates of LAB were identified using a biochemical test referring to the book Identification of Cowan and Steel 1992. Isolation of LAB using pour plate using MRS Agar media enriched with CaCO3 1%. Antibacterial activity test using Kirby Baurer method with disc diffusion technique. The results of the characterization of LAB contained in the intestines of vannamei shrimp were obtained as many as four isolates. Antibacterial activity test of LAB against Vibrio harveyi obtained the highest inhibition zone, namely isolate 2 with a diameter of 4.25 ± 1.77 mm, and was included in the weak category because it was less than 5 mm. The results of bacterial identification using biochemical tests showed that the three isolates were bacteria from the genus Pediococcus and one isolate was a bacterium from the genus Gemella.