Activity of jamblang leaf extract (Syzygium cumini L.Skeels) with various solvents against clinical isolate bacteria

Abstract

Jamblang (Syzygium cumini) is a plant that has uses for traditional medicine and has the potential to be developed as an alternative to new antibiotics. This study is to determine the phytochemical content and activity of jamblang leaf extract against Methicillin-Resistant Staphylococcus aureus bacteria. Extraction of Simplicia powder was carried out using the multilevel maceration method using various solvents such as n-hexane, ethyl acetate, and methanol. Qualitative phytochemical screening and GC-MS analysis. The activity test used the disc diffusion method with a test concentration of 1.25:2.5;5;10;20; and 30%. The results of the phytochemical qualitatively shows that n-hexane extract containing alkaloids, flavonoids, and steroids. The ethyl acetate extract contains alkaloids, saponins, and steroids, while the methanol extract contains alkaloids, flavonoids, tannins, saponins,and triterpenoids. The result from the GC-MS analysis shows that the active fraction of jamblang methanol extract contains compounds with the largest components being HEXADECANOIC ACID, (7E,9E)-Dodecadien-1ol, ALPHA-EUDESMOL, and Guaiol. The activity test results showed that the n-hexane extract formed an inhibition zone of 10.60 mm, the ethyl acetate extract formed an inhibition zone of 11.50 mm while the methanol extract formed an inhibition zone of 20.80 mm at a concentration of 30%. Jamblang leaf methanol extract has a high potential as an antibacterial agent against MRSA clinical isolates.