Activity of isolated specific bacteriophage in treatment of chronic osteomyelitis induced by multiple drug resistance Pseudomonas aeruginosa in Rabbits

Sarhan Rashid Sarhan

doi:10.30539/iraqijvm.v41i2.64

Abstract

This study was conducted to find out the possibility of using the Pseudomonas aeruginosa specific-bacteriophage as an alternative to antibiotics in treatment of chronic osteomyelitis in rabbits by injection of Pseudomonas aeruginosa suspension in tibia. The current study included an isolation of bacteriophage from sewage water by using agar overlay method and also an isolation of the bacteria from patients suffering from post-traumatic bone infection. The second experiment was in-vivo evaluation of phage activity in treatment of chronic osteomyelitis in rabbits. All animals of infected groups with Pseudomonas aeruginosa before treatment exhibited histopathological changes after 35 days of infection, the infected groups showed chronic osteomyelitis represented by sever chronic inflammatory cells infiltrates mainly lymphocytes, and macrophages and hemorrhage between bone trabeculae, also some sections showed extensive fibrosis in the marrow spaces. The treated group with P. aeruginosa specific – bacteriophage (1.5 ×107) PFU/ml for 10 days showed early repair elucidated by presence whorls of chondrocytes, and also the presence of multiple osteoblasts indicated bone formations. Also the presence of extensive fibrosis in the marrow space with present of osteoblasts indicated bone formations and repair.

Highlights

Introduction osteonecrosisIt is associated with formationOsteomyelitis is an inflammatory process of a large area of devascularized dead bone, a of the entire bone including the cortex and the sequestrum (6)
Staphylococcus species, followed by Osteomyelitis is a disease in transition, with Enterobacteriaceae and Pseudomonas species ongoing changes in predisposing factors, (7)
Patients with negative bacteria Pseudomonas aeruginosa certain conditions such as intravenous drug and Enterobacteriaceae have grown in users like heroin, cocaine, methamphetamines important causative agents due to the and opiates, decubitus ulcers, surgery, trauma, increasing number of orthopedic surgery with immunosuppression, immune diseases, diabetes mellitus, number of high-energy traumas associated smoking, malnutrition, malignancy, extremes with open fractures, as a consequence of traffic of age, chronic hypoxia, and renal or hepatic accidents and war injuries (9)

Summary

Introduction

Introduction osteonecrosisIt is associated with formationOsteomyelitis is an inflammatory process of a large area of devascularized dead bone, a of the entire bone including the cortex and the sequestrum (6). The most common pathogens periosteum, accompanied by bone destruction responsible for osteomyelitis in humans are and caused by an infecting organism (1). Haemolytic streptococci are causative organisms and treatment (2). Patients with negative bacteria Pseudomonas aeruginosa certain conditions such as intravenous drug and Enterobacteriaceae have grown in users like heroin, cocaine, methamphetamines important causative agents due to the and opiates, decubitus ulcers, surgery, trauma, increasing number of orthopedic surgery with immunosuppression, immune diseases (i.e. the use of implants and, especially, the rising rheumatoid arthritis), diabetes mellitus, number of high-energy traumas associated smoking, malnutrition, malignancy, extremes with open fractures, as a consequence of traffic of age, chronic hypoxia, and renal or hepatic accidents and war injuries (9). There are three failure are at increased risk of osteomyelitis classes of antibiotic resistance in P

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Conclusion