Abstract
Candida spp. resistant to commercially available antifungals are often isolated from patients with oral candidiasis, a situation that points to the need for the development of new therapies. Thus, we evaluated the activity of Fusarium oxysporum-based silver nanoparticles (AgNPs) on Candida spp. isolated from denture stomatitis lesions. Candida isolates were molecularly identified and submitted to susceptibility assays using AgNPs and commercial fungicides. The interference on biofilm formation and the mechanisms of action of AgNPs on Candida spp. were also investigated. Scanning electron microscopy was used to evaluate the morphology of AgNP-treated Candida. Candida albicans was the most frequent species isolated from denture stomatitis cases. All Candida spp. were susceptible to AgNPs at low concentrations, except Candida parapsilosis. AgNPs caused surface damage, cell disruption, and biofilm formation inhibition. The ergosterol supplementation protected C. albicans against the AgNP action. AgNPs are effective against Candida spp. and can be faced as a promising new therapeutic agent against oral candidiasis.
Highlights
Introduction published maps and institutional affilDenture stomatitis is an infection of the oral cavity characterized by inflammation and erythema, and predisposing conditions increase the ability of opportunistic Candida fungi to cause oral candidiasis [1]
Considering the potential of biogenic AgNPs as antimicrobial agents, we aimed to evaluate their antifungal activity against Candida spp. isolated from patients with prosthetic stomatitis
The twenty Candida isolates from denture stomatitis lesions were identified through the sequencing of large subunit (LSU) and ITS gene fragments
Summary
Denture stomatitis is an infection of the oral cavity characterized by inflammation and erythema, and predisposing conditions increase the ability of opportunistic Candida fungi to cause oral candidiasis [1]. This infection is caused by Candida albicans and by other non-Candida albicans (NCA) species, such as Candida parapsilosis, Candida tropicalis and Candida glabrata [2]. Candida species are competent biofilm-forming microorganisms, and these structures are correlated with an enhanced resistance to antifungals [5]. The inappropriate use of antifungals has resulted in the emergence of multidrug-resistant fungi [6]. The use of alternative technologies, such as metallic nanoparticles, has been considered in the last few years since they iations
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