Abstract
Kinetic analysis of erythrocyte δ-aminolevulinic acid dehydrase (δ-ALAD) from female cynomolgus monkeys revealed differences in pH optimum and Michaelis constants according to their exposure to lead. In vitro incubation of δ-ALAD with 5 mM dithiothreitol (DTT) or 100–200 μM zinc resulted in an enhanced enzyme activity being expressed. These effects were additive. Activation with DTT or zinc resulted in the abolition of pH differences between control and exposed animals and revealed an increased quantity of enzyme in exposed animals. δ-ALAD in control monkeys was observed to be very sensitive to inhibition by lead in vitro with an apparent inhibition constant ( K 1 ) of 0.12 μM. The effect of lead on monkey δ-ALAD enzyme kinetics is similar to that seen with human samples and thus is a useful model for measuring biological response to lead exposure.
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