Abstract
Background Males of the order Squamata have similar structure to the seminal vesicles of mammals called Renal Sexual Segment (RSS). The RSS regions are hypertrophied kidney ducts, androgen-dependent, responsible for providing secretions (complex of glycogen, mucopolysaccharides, mucoproteins and lipids) that nourishes and keeps the sperm during copulation [1,3], acting accessorily on reproductive cycle, once when mixed with sperm, and nutrient function, also act as activating agent of sperm transferred to the cloaca in the female during intercourse [2,3]. Valverde (personal communication, April 6, 2010) tested the crude extract of the renal sexual segment Crotalus durissus in bovine semen and the results showed a significant increase in sperm motility and vigor of the samples, but this activity was lost over time storage of the extract. The promising data in this study aimed to evaluate the effect of the addition of crude extract of the RSS from C. durissus on spermatic kinematics of thawed dog semen.
Highlights
Males of the order Squamata have similar structure to the seminal vesicles of mammals called Renal Sexual Segment (RSS)
Dog semen samples (n = 3) cryopreserved in tris-yolkglycerol (7%) were thawed (37oC/30s) and divided into three groups: G1 = semen + 48μg/mL extract unheated, G2 = semen + 48μg/mL heated extract (60oC/30min) and G3 = semen
The extract used was diluted in Tris buffer (0.25M Tris, 0.09M citric acid, fructose 0.06M, pH 6.8) to 0.2 mg/mL
Summary
Cristiane Scavuzzi Moura1*, Chirlane Castro Silva, Sildivane Valcácia Silva, Ellen Cordeiro, Bento Silva, Ranilson S Bezerra, Maria Madalena Pessoa Guerr. From 5th Congress of the Brazilian Biotechnology Society (SBBIOTEC) Florianópolis, Brazil. From 5th Congress of the Brazilian Biotechnology Society (SBBIOTEC) Florianópolis, Brazil. 10-14 November 2013
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