Activity of Aegle marmelos (L) Correa (Buah Maja) Ethanol Extract to Multiresistant Eshecichia coli Clinical Isolates from Urinart Tract Infection Patients

Abstract

The increase in resistance of Escherichia coli bacteria that caused urinary tract infections to antibiotics narrows the selection of effective treatment which encourages the development of treatment therapies using natural ingredients. Maja fruit [Aegle marmelos (L.) Correa] is a plant that has shown antibactericidal activity against gram-negative bacteria, one of which is Escherichia coli. This study aimed to determine the antibacterial activity of fruit Maja extract, minimum inhibition concentration (MIC) and the minimum bactericidal concentration (MBC) of fruit Maja extract and the comparative value of gentamycin against Escherichia coli resistance isolated from urinary tract infection patients. The methods are extraction of Maja fruit by using 96% ethanol, agar diffusion method with paper disc and determination of MIC and MBC using microdilution method. The results showed that the ethanol extract of Maja fruit had antibacterial activity with value of MIC50 as 1400-1500 μg/ml, the value of MIC90 was 1600 μg/ml and the value of MBC was in the range concentration of 1700-2000 μg/ml against E. coli isolate I. Against E. coli isolates II, value of MIC50 was in concentration of 2900 μg/ml, MIC90 was in concentration 3000 μg/ml and the value of the MBC is in range 3150-3500 μg/ml. The comparative values of the antibacterial activity of gentamycin on fruit maja extract are 1:5.871 x 103.