Activity of 1-aminocyclopropane carboxylic acid synthase and growth of mustard (Brassica juncea) following defoliation

Abstract

To examine the possible relationship between the activity of 1-aminocyclopropane carboxylic acid synthase (ACS; EC 4.4.1.14) and growth of mustard (Brassica juncea L.), ACS activity, ethylene and plant growth were studied in the presence of ACS activity modulators in no-defoliation and defoliated plants. Growth of plants was greatest when subjected to defoliation of 50% lower leaves in the plant axis compared to defoliation of 25% lower leaves or no-defoliation. The activity of ACS in no-defoliation and defoliated plants was correlative with growth of plants. ACS activity and ethylene evolution in no-defoliation plants treated with 10 μM indole-3-acetic acid (IAA) and defoliated plants treated with water were equal and resulted in maximum plant growth. On the contrary, the application of 10 μM IAA on defoliated plants resulted in the increase in ACS activity and ethylene evolution to an extent that inhibited the growth. The application of 100 μM IAA on no-defoliation and defoliated plants increased ACS activity and ethylene evolution maximally and proved inhibitory for the plant growth. The association of ACS activity, ethylene evolution and growth of plants was further substantiated with the use of 50 μM aminoethoxyvinyl glycine (AVG) applied alone or in combination with 10 or 100 μM IAA. The application of AVG resulted in the inhibition of ACS activity and the growth of no-defoliation or defoliated plants. The results indicate that there exists a correlation between ACS activity, ethylene and the growth of mustard plants.