Activity and expression of human telomerase in normal and malignant cells in gastric and colon cancer patients.

Abstract

The reactivation of telomerase is believed to play an important role in immortalization and carcinogenesis. To investigate the expression of three components of the telomerase complex (hTR, hTERT and TP1), along with telomerase activity in malignant and normal cells. Cells were isolated from gastric and colon cancer, and from normal mucosa from the stomach and colon of participating patients. Expression of hTERT, hTR and TP1 has been studied by the reverse transcriptase polymerase chain reaction (PCR) technique. The telomerase repeat amplification protocol and PCR enzyme-linked immunosorbent assay were used for analysis of telomerase activity. All telomerase components were consistently expressed in colon and gastric cancer cells. Neoplastic RNA produced consistently very strong amplification signals either for hTR, hTERT or TP1. The expression of hTR was observed in RNA isolated from all normal mucosa samples and from peripheral blood lymphocytes. The expression of TP1 and hTERT has been found in the majority of normal cells; however, the amplification signals produced were usually much weaker than in malignant cells. The limiting dilution experiments indicated that the cancer cells have at least 100-fold higher telomerase activity and at least 25-fold higher TP1 and hTERT expression in comparison to normal cells. It can be concluded that all the cancer cells tested have higher telomerase expression and activity than normal cells. Therefore, telomerase can be a good cancer marker, provided that quantitative analysis is carried out.