Activities of 3-oxo acid-CoA transferase and acetoacetyl-CoA synthetase in brain and liver of the developing chick

Abstract

The 3-oxo acid-CoA transferase and acetoacetyl-CoA synthetase activities were estimated in subcellular fractions isolated from the cerebral hemispheres, the optic lobes, the cerebellum and the liver of the chick between the twentieth day of embryonic life and the thirtieth day of postnatal development. In the 3 areas of chick brain, the tranferase activity increases during the whole postnatal development. Transferase is located both in mitochondria and microsomes unlike in the rat brain where this enzyme is specifically mitochondrial. There is no detectable activity of synthetase in the chick brain. These differences in enzyme localization imply that acetoacetate is converted into acetoacetyl-CoA only by 3-oxo acid-CoA transferase in the mitochondria and cytoplasm of the chick brain, whereas, in the rat brain, this reaction is performed by 3-oxo acid-CoA transferase in mitochondria and by acetoacetyl-CoA synthetase in the cytosol. There is no detectable activity either for transferase or for synthetase in the chick liver.