Actividad peroxidasa y concentración de proteínas en Phaseolus vulgaris l. tratado con preparaciones homeopáticas

Tarita Cira Deboni,Egabrieli Garbin,Gabriela De Melo Santiago,Júlia Carolina Ghizzoni,Alexandre Giesel,Pedro Boff,Alexandre Copatti Loregian,Cláudia Petry,Denise Cargnelutti

doi:10.33448/rsd-v10i9.18457

Abstract

The objective of this work was to evaluate peroxidase activity and protein concentration in the defence system expression of common bean Phaseolus vulgaris L., submitted to different potencies of homeopathic preparations. The treatments were composed by: Arsenicum album, Staphisagria, Silicea and Sulphur, in the 6, 12 and 18 CH potencies, compared to the controls. The bean seeds were sown in pots which were kept in a greenhouse and the treatments were applied to them at 1% in distilled water since the sowing day, with intervals of 15 days until the R7 stage. The first evaluation was performed at 15 e 60 days after sowing, after artificial mechanical damage. Protein concentration and the activity of guaiacol peroxidase (POD) and ascorbate peroxidase (APX) enzymes were determined. Differences between treatments and controls were estimated by Glass Delta effect size analysis. Staphisagria 12CH induced greater increase in protein concentration on bean leaves. The Induction on APX and POD activities were observed in leaves treated with Sulphur in all tested potencies. The Glass Delta effect showed undulatory behaviour, sometimes acting positively, sometimes negatively, throughout all evaluations. Homeopathic preparations have the potential to induce biochemical defence mechanisms in beans, acting as resistance elicitors.

Highlights

The homeopathy, science and healing art proposed by Hahnemann in the XVIII century, was developed on the basis of healing by the like, Similia Similibus Curentur, the pathogenetic experimentation in the healthy individual, minimal medicine doses and single medicine use (Andrade and Casali 2011; Teixeira 2011)
The surveys are grouped into three categories: a) healthy plant models, aiming to investigate questions related to homeopathic potencies and pathogenetic assays; b) phytopathological models, which apply homeopathy in the management of plant diseases and pests, without use restriction in the systems mentioned in the Organics Law, in Brazil; and c) models using plants subjected to abiotic stresses (Betti et al 2009; Majewsky et al 2009; Jäger et al 2011, 2015)
The objective of this work was to evaluate protein content and peroxidase activity in the expression of the defense system of common bean Phaseolus vulgaris L. submitted to different potencies of homeopathic preparations

Summary

Introduction

The homeopathy, science and healing art proposed by Hahnemann in the XVIII century, was developed on the basis of healing by the like, Similia Similibus Curentur, the pathogenetic experimentation in the healthy individual, minimal medicine doses and single medicine use (Andrade and Casali 2011; Teixeira 2011). Several groups of researchers in Brazil and worldwide have been working on the homeopathy effects and on the outcomes of highly diluted aqueous substances in plants (Santos and Pontes 2013; Teixeira and Carneiro 2017; Ücker et al.2018). The surveys are grouped into three categories: a) healthy plant models, aiming to investigate questions related to homeopathic potencies and pathogenetic assays; b) phytopathological models, which apply homeopathy in the management of plant diseases and pests, without use restriction in the systems mentioned in the Organics Law, in Brazil; and c) models using plants subjected to abiotic stresses (mineral toxicity, salinity, pH, etc.) (Betti et al 2009; Majewsky et al 2009; Jäger et al 2011, 2015). Homeopathic preparations can act as resistance inductors to insects and pests, assisting in maintaining plant productivity through the secondary metabolites production (Moreno 2017)

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