Abstract

Milk whey is largely discarded from the food industry. Therefore, milk whey is an attractive bioresource for microbial value-added protein production. Demand for transglutaminase (TG), which catalyzes crosslinking of proteins, is increasing in various industries. Recently, a Saccharomyces cerevisiae host strain that synthesizes a large amount of glutathione has been developed for active TG production because glutathione is an activator of TG. In this study, β-D-galactosidase was introduced into this strain to produce active Streptoverticillium mobaraense TG from skimmed milk whey hydrolysate.

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