Abstract
Cobra (Naja haje) venom detoxified by gamma radiation (15KGy or 25KGy) as toxoid for active immunization of rabbits following a short schedule ( on day 0, 7,21) of immunization with complete Freund's adjuvant. Effective neutralization of venom toxin by immune sera of rabbits was observed. The presence of antibody in the immune sera was detected by immuno-diffusion test. The non-irradiated and the two dose levels gamma irradiated Naja haje venom, against the antivenin antibody produced with non-irradiated venom or against antivenin antibody produced with 15 KGy gamma irradiated venom all showed similar patterns. Also there was no change in the titer of antivenin solution obtained from the rabbit immunized with 15KGy irradiated venom , as compared with that antivenin solution obtained from rabbit immunized with non-irradiated venom. Sera of rabbits immunized with 15KGy irradiated venom toxoid (15KGy toxoid antiserum) neutralized venom lethality, 95% of protease activity and 50% of phospholipase A2.
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