Active Fraction as Anti-obesity by in Vitro toward Pancreatic Lipase Activity

Abstract

Fruit extract of asamgelugur (Garciniaatroviridis) can be used as an anti-obesity by in vitro against pancreatic lipase activity. The pancreatic lipase plays a role in degradation of lipids into fatty acids, as pancreatic lipase activity is hampered, the absorption of monoglycerides in the human body decreases. The purpose of the research was to identify and evaluate the Garciniaatroviridisfruit fraction as pancreatic lipase inhibitor. The extracted fruit with percolation method showed an inhibitory activity of 44.16% at 500 µg/mL. Xenical® as the positive control exhibited an inhibitory activity of 43.65% at 25 µg/mL. The best result from partitioning was using BuOH solvent with inhibitory activity of 56.42% at 100 µg/mL, whereas from Xenical® was 50.35% at 50 µg/mL. The best fraction from BuOH fractionation eluting with CH2Cl2:MeOH revealed an IC50 value of <10 µg/mL, or was better than Xenical®. Therefore, E fraction from BuOH solvent of garciniaatroviridisextract is potential as a pancreatic lipase inhibitor.