Abstract
By using Monte Carlo (MC) simulations, we have shown that the active forces generated by (NMIIA) motor domains bound to F-actin may partially control the endovesiculation of the red blood cell (RBC) membrane. The myosin generated active forces favor pancake-like (torocyte) RBC endovesicles with a large flat central membrane region and a bulby periphery. We suggest that the myosin generated active forces acting on the RBC membrane in the direction perpendicular to the membrane surface towards the interior of the RBC may influence also other RBC shape transformations and the stability of different types of RBC shapes and should be therefore considered in the future theoretical studies of the RBC vesiculation and shape transformations.
Highlights
A biological membrane forms a physical boundary between the inner volume of a biological cell and the external medium, as well as, within the cell, between the lumens of intracellular organelles and cytosol
By using Monte Carlo (MC) simulations, we have shown that the active forces generated by (NMIIA) motor domains bound to F-actin may partially control the endovesiculation of the red blood cell (RBC) membrane
The myosin generated active forces favor pancake-like RBC endovesicles with a large flat central membrane region and a bulby periphery
Summary
A biological membrane forms a physical boundary between the inner volume of a biological cell and the external medium, as well as, within the cell, between the lumens of intracellular organelles and cytosol. The results of theoretical modeling indicated that torocyte RBC endovesicles can be mechanically stabilized by non-homogeneous lateral distribution of laterally mobile anisotropic membrane inclusions, like for example by anisotropic detergent-membrane component complexes.[58,59] It was further shown in 2019 that the mechanical stability of torocyte (pancake-like) closed membrane vesicle shapes can be explained by the coupling of the curved isotropic membrane inclusions and active (cytoskeletal) forces.[55] Until recently,[60] it was believed that the active forces are absent in the mechanisms of the determination of the RBC shape and vesiculation, as discussed above It has been shown in 2018 that nonmuscle myosin IIA (NMIIA) motor domains may generate tension in spectrin-F-actin in a 2-dimensional RBC membrane skeleton and in this way partially control the RBC shape.[60] The role of NMIIA contractility in generating tension in the RBC network and partially controlling the RBC shape was in the past completely neglected, until recently.[60] The length of NMIIA filament is around 200 nm.[60].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
