Abstract
Chemical analysis of methanol extract of flowers and leaves of Bougainvillea alba led to isolation of four compounds identified as 3’, 4’, 6 - trimethoxy, 5 – hydroxyl 7-prenyl 3-O- (feruloyl (1→2) - rhamnpyranosyl) flavone[1], 3-O β-D-glucopyranosyl oleanolic acid 28-O-β-D-glucopyranosyl (1→3”) β-D-glucopyranosyl (1→3”’) α-L–xylopyrenoside [2], Lepidolide [3], E feruloyl 3-O- Coumaroyl α-L Rhamnopyranoside [4]. In addition, two oil fractions, the major identified components of oil are Elemene, Lanceol , Nerolidol , Methyl palmitate and Abietatriene. Cytotoxic activity of methanol extract of both leaves and flowers of <em>Bougainvillea alba</em>, isolated compounds and oil fractions were determined using the SRB Assay on human tumor cell line (Hepatocyte generation 2, HepG2). The isolated compounds showed cytotoxic activity at LC50 15.2, 17.2, 15.6 and 2.82 μg/ml for compound 1,2, 3 and 4 respectively, while the two oil fraction showed activity at 2.67 μg/ml.
Highlights
Plant MaterialFlowers and leaves of Bougainvillea glabra ‘Alba’ (Bunga Kertas) were collected from of Banha Agriculture university gardens, The plant was kindly identified by Mrs Terasa Labib, General Manager and head of plant Taxonomy in El-Orman Botanical Garden, Giza, Egypt
sulforhodamine B (SRB) is a bright–pink amino oxanthene dye with two sulfonic groups that bind to basic amino acid residues under mild acidic conditions, and dissociate under basic conditions
Tris base 10 mM (PH 10.5): It was used for SRB dye solubility. 121.1 gm of tris base was dissolved in 1000 ml of distilled water and PH was adjusted by HCl acid (2 M)
Summary
Flowers and leaves of Bougainvillea glabra ‘Alba’ (Bunga Kertas) were collected from of Banha Agriculture university gardens, The plant was kindly identified by Mrs Terasa Labib, General Manager and head of plant Taxonomy in El-Orman Botanical Garden, Giza, Egypt. The flowers and the leaves of the plant were isolated, dried separately in shade, finely powder with an electric mill
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