Active anti-acetylcholinesterase component of secondary metabolites produced by the endophytic fungi of Huperzia serrata

Zhejian Wang,Chengchen Tang,Zhibi Hu,Lili Wang,Guixin Chou,Zhao Ma,Wankui Li

doi:10.1016/j.ejbt.2015.08.005

Abstract

Background: An endophytic fungus lives within a healthy plant during certain stages of, or throughout, its life cycle. Endophytic fungi do not always cause plant disease, and they include fungi that yield different effects, including mutual benefit, and neutral and pathogenic effects. Endophytic fungi promote plant growth, improve the host plant's resistance to biotic and abiotic stresses, and can produce the same or similar biologically active substances as the host. Thus, endophytic fungal products have important implications in drug development. Result: Among the numerous endophytic fungi, we identified two strains, L10Q37 and LQ2F02, that have anti-acetylcholinesterase activity, but the active compound was not huperzine A. The aim of this study was to investigate the anti-acetylcholinesterase activity of secondary metabolites isolated from the endophytic fungi of Huperzia serrata . Microbial cultivation and fermentation were used to obtain secondary metabolites. Active components were then extracted from the secondary metabolites, and their activities were tracked. Two compounds that were isolated from endophytic fungi of H. serrata were identified and had acetylcholine inhibitory activities. In conclusion, endophytic fungal strains were found in H. serrata that had the same anti-acetylcholinesterase activity. Conclusion: We isolated 4 compounds from the endophytic fungus L10Q37, and make sure that there are two new compounds, compounds S1 and S3. And all 6 compounds are new compounds isolated from LQ2F02. From the view there anti acetylcholinesterase activity, S5 is the best, other compounds' anti acetylcholinesterase activity was not good, and compared with huperzine A there are a larger gap.

Highlights

Alzheimer's disease is a neurodegenerative disease of the central nervous system
A study found that of the 10–15% of elderly people with different degrees of dementia, approximately 60–70% of the cases are due to Alzheimer's disease [2]
We studied metabolites of the endophytic fungi L10Q37 and LQ2F02, which were isolated from H. serrata, to obtain acetylcholinesterase inhibitors

Summary

Introduction

Alzheimer's disease is a neurodegenerative disease of the central nervous system. The first clinical manifestation is recent memory dysfunction, which is followed by persistent intellectual impairment, loss of judgment and reasoning abilities, aphasia, and movement dysfunction [1]. Components that inhibit cholinesterase activity could be extracted from the endophytic fungi of H. serrata instead of extracting active components from plants. This allowed us to identify new compounds with anti-acetylcholinesterase activities, which may solve the H. serrata resource exhaustion crisis. We studied metabolites of the endophytic fungi L10Q37 and LQ2F02, which were isolated from H. serrata, to obtain acetylcholinesterase inhibitors. Result: Among the numerous endophytic fungi, we identified two strains, L10Q37 and LQ2F02, that have anti-acetylcholinesterase activity, but the active compound was not huperzine A. The aim of this study was to investigate the anti-acetylcholinesterase activity of secondary metabolites isolated from the endophytic fungi of Huperzia serrata. Other compounds' anti acetylcholinesterase activity was not better compared with huperzine A

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Conclusion