Abstract
1. The haemolymph of Periplaneta americana contains the proenzyme prophenoloxidase (EC 1.14.18.1) which was activated by incubation of the haemolymph with various organic solvents. Their effectiveness was acetone > methanoi > ethanol > propanol > butanol. 2. Activated phenoloxidase oxidized diphenols, d- and l-Dopa, pyrocatechol, epinephrine and norepinephone and the triphenol pyrogalcol, but showed no activity towards tyrosine or other monophenols. 3. Various methods of drawing haemolymph were evaluated for their effectiveness in recovering activatable phenoloxidase activity. Acetic acid pretreatment of the whole insect prior to collecting haemolymph was found to be the best, apparently due to its effectiveness in inhibiting haemocyte coagulation. 4. The activated enzyme was inhibited most effectively by phenylthiourea and had a pH optimum of 6.
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