Activation of the protein synthetic capacity of rat liver cell sap by amino-acids.

Abstract

CHANGES in the availability of amino-acids have marked effects on the rate of protein synthesis in rat liver1–4. A high amino-acid concentration in the perfusion5 or incubation6 medium is needed to observe an effect of growth hormone in vitroon incorporation of precursors into protein and RNA of isolated hepatic tissue. We report here changes in the ability of cell-free systems to incorporate amino-acids into acid-insoluble material in vitrowhen they were prepared from slices incubated in various concentrations of amino-acids.