Activation of Radioadaptive Response via Injected Serum of Irradiated Rats with Low Doses of Ionizing Radiation

Abstract

Introduction: One of the protective concepts in the interpretation of radiation damage in radiobiology is the radioadaptive response. The objective of this present report is to evaluate the induction of radioadaptive response in male rats by injection of pre-irradiated serum with low doses X-radiation intraperitoneally. Materials and Methods: 80 male adult Wistar rats were randomly divided into 6 groups include control (sham exposed), serum control (injected blood serum of non-irradiated rats), low dose(100cGy), low dose/lethal (100cGy then 8Gy), serum/lethal(injected blood serum of irradiated rats+8Gy). and lethal(8Gy). In this study, we have monitored the end points of survival rate; the first stages, rats were exposed to low doses of radiation (100cGy), then, the serum was injected intraperitoneally under sterile conditions and under the laminar hood 6hour after low dose exposure. The Kaplan Meier Survival Curve was used to evaluate survival rate in the studied groups p< 0.05 is considered as significant between groups. Results: We found that there was a significant difference between the survival rates in different groups. There were no deaths in the control, serum and low dose groups at 13, 18, and 22 days after the start of the experiment and survival rates was 100%. Statistically significant difference between low dose/lethal and low dose/serum, low dose/lethal and lethal, and low dose /serum and lethal (p=0.001). Likewise, the data confirm that a statistically significant difference between survival rates of the rats in control and test groups was observed (p=0.001). Conclusion: In conclusion, this Study showed that exposure to low dose radiation and then serum injection to adapting group may induce a significant survival adaptive response.