Activation of phospholipases by N‐acetylchitooligosaccharide elicitor in suspension‐cultured rice cells mediates reactive oxygen generation

Abstract

The generation of reactive oxygen species (ROS) is a central component of the elicitor‐induced defence reactions in cultured cells as well as the resistance responses of plants to pathogen challenge. We show that N‐acetylchitooligosaccharide elicitor induces rapid and transient activation of phosphatidylinositol‐specific phospholipase C (PI‐PLC) and that phosphatidylcholine‐specific phospholipase D (PC‐PLD) in suspension‐cultured rice cells and their products, phosphatidic acid (PA) and diacylglycerol (DG), especially the former, play an important role in the elicitor‐induced ROS generation based on the following observations: (1) the amount of PA and DG in rice cells was rapidly increased by the elicitor treatment. (2) Elicitor‐induced activation of PI‐PLC and PC‐PLD in the membrane fraction was confirmed by the analysis of enzymatic products from radio‐labelled phospholipids as well as by 1‐butanol (1‐ButOH)‐specific formation of phosphatidylbutanol (PtdBut) (for PC‐PLD). Inhibitors of these phospholipases at least partly inhibited the elicitor‐induced ROS generation. (3) Exogenously applied PA and DG could induce ROS generation in the rice cells in the absence of the elicitor. (4) PA phosphohydrolase (PAPH) and diacylglycerol kinase (DGK) activities, which catalyse the conversion of PA and DG with each other, are present in the rice cells and the inhibitors of these enzymes inhibited/stimulated the elicitor‐induced ROS generation depending on the direction of the PA accumulation. These results indicate the important role of PI‐PLC/PC‐PLD and their products, especially PA, in the signal transduction cascade downstream of the N‐acetylchitooligosaccharide receptor.