Activation of Orange II Decoloration Reaction with Peroxidase Under Low Temperature

Abstract

Decoloration of Orange II catalyzed horseradish peroxidase (HRP) with activator as p-iodophenol, sodium nonanoyloxybenzenesulphonate (NOBS) and sodium p-phenolsufonate under low temperature was studied kinetically. Decoloration rate constant (k) that can prevent dye transfer of Orange II (k = 2.0 min-1) was not obtained in 3.4 × 10-8 M HRP without activator. Optimum condition using p-iodophenol was pH 8.9 Carmody buffer and 5.0 × 10-5 M p-iodophenol. Optimum pH using NOBS and sodium p-phenolsufonate were 10.1 and 10.3 respectively. Decoloration rate constant decreased with increase in the concentration NOBS. k = 2.0 min-1 was not reached unless high sodium p-phenolsulfonate. Therefore, it was found that the use of p-iodophenol appears optimal. Decoloration reaction of Orange II using HRP with p-iodophenol and sodium percarbonate to prevent dye transfer was optimized under low temperature. The activation energy of decoloration of Orange II using HRP with p-iodophenol was 1/2 - 1/3 of those without activator and using sodium percarbonate.