Activation of NF-κB contributes to production of pig-major acute protein and serum amyloid A in pigs experimentally infected with porcine circovirus type 2

Abstract

Acute phase proteins (APPs) have protective and regulatory roles in the inflammatory response. Previous studies indicate that APPs in serum change after pigs are infected with porcine circovirus type 2 (PCV2), but the mechanisms underlying APP production have remained unclear. In this present study, 35-day-old pigs were challenged with PCV2 and responses compared to an uninfected control group. To investigate the concentrations of APPs in serum and the activity of NF-κB in the liver, five pigs in the PCV2-infected group were euthanized at 14, 21 and 35days post inoculation (dpi) while four pigs were sacrificed in the control group at 0, 14, 21 and 35days, respectively. The concentrations of pig-major acute protein (Pig-MAP), C-reactive protein (CRP) and serum amyloid A (SAA) in infected animals were increased at 14 and 21dpi, while the concentration of alpha-1 acid glycoprotein (AGP) was lower at 35dpi, indicating that PCV2 induced the production of APPs. Moreover, the DNA binding activity of NF-κB and expression levels of NF-κB p65 subunit (NF-κB p65) from the cytoplasm to nucleus were increased at 14 and 21dpi in the liver of infected pigs, while the phosphorylation of IκBα (p-IκBα) in the liver was also increased at 21dpi. This demonstrated that PCV2 infection induced the activation of NF-κB. Both SAA and Pig-MAP concentrations correlated significantly with expression levels of NF-κB p65, indicating that activation of NF-κB contributes to the production of SAA and Pig-MAP.