Abstract
The effect of prostaglandins on neutrophil activation has been studied using the human promyelocytic leukemic cell line HL-60, differentiated with dimethyl sulfoxide (DMSO). Prostaglandin E 2 (PGE 2) directly stimulated HL-60 cell aggregation with an EC 50 value of 30 nM. Studies with prostanoid receptor-selective agonists suggest that the activation of HL-60 cells by PGE 2 was mediated via the EP 2 receptor. Human neutrophils did not aggregate in response to PGE 2, but PGE 2 inhibited the N-formyl-methionyl-leucyl-phenylalanine (FMLP)-stimulated increase in intracellular calcium ([Ca 2+] i) in both neutrophils and HL-60 cells. In contrast with the aggregation response to FMLP, the aggregation response of HL-60 cells to PGE 2 was independent of extracellular calcium and did not involve mobilization of intracellular calcium.
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