Abstract
We studied the effect of adenosine on Na+/Ca2+ exchange activity in ewe heart ventricular sarcolemmal vesicles. Adenosine was found to stimulate Na+/Ca2+ exchange activity in a dose-dependent manner from 0.1 nM to 10 microM, with maximal stimulation (40%) at 0.1 microM adenosine. The Vmax of Na+/Ca2+ exchange was increased, but the Km for Ca2+ was not altered. The effect of adenosine was specific since 1 microM adenine, inosine, and guanosine led to less than 15% stimulation, and adenosine diphosphate had no effect. Caffeine antagonized the activation of Na+/Ca2+ exchange by adenosine, and the order of potency of adenosine analogs was N6-(L-2-phenylisopropyl)adenosine = N6-cyclohexyladenosine = 5'-(N- ethylcarboxamido)adenosine much greater than N6-(D-2-phenylisopropyl)adenosine, indicating the involvement of A1 subclass receptors. The effect of adenosine was mimicked by guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) and blocked by pertussis toxin treatment. Taken together, these results suggest that A1 subclass receptors coupled to a pertussis toxin-sensitive G protein mediate the activation of Na+/Ca2+ exchange activity by adenosine. We conclude that the negative inotropic effect of adenosine in ventricular muscle, antagonistic toward cyclic AMP, may involve activation of Na+/Ca2+ exchange.
Highlights
From the Institut National de la Sant6 et de la Recherche Mkdicale, Unit6 99, H6pital Henri Mondor, 94010 Criteil, France
We studied the effect of adenosine on Na+/Ca’+ exchange activity in ewe heart ventricular sarcolemmal vesicles
The effect of adenosine was specific since 1 MM adenine, inosine, and guanosine led to less than 15% stimulation, and adenosine diphosphate had no effect
Summary
From the Institut National de la Sant et de la Recherche Mkdicale, Unit , H6pital Henri Mondor, 94010 Criteil, France. We studied the effect of adenosine on Na+/Ca’+ exchange activity in ewe heart ventricular sarcolemmal vesicles. The effect of adenosine was specific since 1 MM adenine, inosine, and guanosine led to less than 15% stimulation, and adenosine diphosphate had no effect. Caffeine antagonized the activation of Na+/Ca’+ exchange by adenosine, and the order of potency of adenosine analogs was N6+2-phenylisopropyl)adenosine. > p-(o-2-phenylisopropyl)adenosine, indicating the involvement of A, subclass receptors. The effect of adenosine was mimicked by guanosine 5’-0-(3-thiotriphosphate) (GTPrS) and blocked by pertussis toxin treatment. We conclude that the negative inotropic effect of adenosine in ventricular muscle, antagonistic toward cyclic AMP, may involve activation of Na+/Ca’+ exchange
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