Activation of MK5/PRAK by the atypical MAP kinase ERK3 defines a novel signal transduction pathway

Abstract

Erratum27 January 2005free access Activation of MK5/PRAK by the atypical MAP kinase ERK3 defines a novel signal transduction pathway Ole-Morten Seternes Ole-Morten Seternes Search for more papers by this author Theresa Mikalsen Theresa Mikalsen Search for more papers by this author Bjarne Johansen Bjarne Johansen Search for more papers by this author Espen Michaelsen Espen Michaelsen Search for more papers by this author Chris G Armstrong Chris G Armstrong Search for more papers by this author Nick A Morrice Nick A Morrice Search for more papers by this author Benjamin Turgeon Benjamin Turgeon Search for more papers by this author Sylvain Meloche Sylvain Meloche Search for more papers by this author Ugo Moens Ugo Moens Search for more papers by this author Stephen M Keyse Stephen M Keyse Search for more papers by this author Ole-Morten Seternes Ole-Morten Seternes Search for more papers by this author Theresa Mikalsen Theresa Mikalsen Search for more papers by this author Bjarne Johansen Bjarne Johansen Search for more papers by this author Espen Michaelsen Espen Michaelsen Search for more papers by this author Chris G Armstrong Chris G Armstrong Search for more papers by this author Nick A Morrice Nick A Morrice Search for more papers by this author Benjamin Turgeon Benjamin Turgeon Search for more papers by this author Sylvain Meloche Sylvain Meloche Search for more papers by this author Ugo Moens Ugo Moens Search for more papers by this author Stephen M Keyse Stephen M Keyse Search for more papers by this author Author Information Ole-Morten Seternes, Theresa Mikalsen, Bjarne Johansen, Espen Michaelsen, Chris G Armstrong, Nick A Morrice, Benjamin Turgeon, Sylvain Meloche, Ugo Moens and Stephen M Keyse The EMBO Journal (2005)24:873-874https://doi.org/10.1038/sj.emboj.7600591 This article corrects the following: Activation of MK5/PRAK by the atypical MAP kinase ERK3 defines a novel signal transduction pathway02 December 2004 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Correction to: The EMBO Journal (2004) 23, 4780–4791. doi:10.1038/sj.emboj.7600489 Due to a typesetting error, Figure 3c of the above article was published incorrectly in print. The correct Figure 3 is reproduced below in its entirety. Figure 1.Amino acids 330–340 of ERK3 are required for interaction with and relocalisation of MK5. (A) COS-1 cells were transfected with expression vectors encoding Myc-tagged full-length, or C-terminal truncation mutants of ERK3 encoding either amino acids 1–340 or 1–330 of the protein. Cell lysates were then mixed with either recombinant GST-MK5 or GST alone. Binding of ERK3 was detected by Western blotting using an anti-Myc monoclonal antibody. (B) HeLa cells were transfected with expression vectors encoding either amino acids 1–330 or 1–340 of ERK3. ERK3 was visualised by staining with an anti-ERK3 antibody and an Alexa 594-coupled secondary (anti-sheep) antibody (red channel in upper panels) and cell nuclei were visualised by DRAQ5 staining (blue channel in lower panels). In all, 100 cells expressing the indicated mutant of ERK3 from each of three independent transfections were counted and the distribution of the ERK3 protein was scored. The results are presented as the % of cells in which the ERK3 protein was predominantly nuclear (N>C) and mean values with associated errors are shown. (C) HeLa cells were cotransfected with vectors encoding EGFP-MK5 and either ERK3 1–330 or 1–340. EGFP-MK5 was visualised directly (green channel in upper panels), ERK3 was visualised by staining with an anti-ERK3 antibody and an Alexa 594-coupled secondary (anti-sheep) antibody (red channel in middle panels) and cell nuclei were visualised by DRAQ5 staining (blue channel in lower panels). A total of 100 cells expressing either EGFP-MK5 alone or coexpressing both EGFP-MK5 and the indicated mutant of ERK3 from three independent transfections were counted and the distribution of MK5 was scored. The results are presented as the % of cells in which EGFP-MK5 was either predominantly nuclear (N>C) or predominantly cytoplasmic (C>N) and mean values with associated errors are shown. In all experiments, several fields of cells were examined and representative images are shown. Download figure Download PowerPoint The Publisher would like to apologise for any inconvenience this may have caused. Next ArticlePrevious Article Read MoreAbout the coverClose modalView large imageVolume 24,Issue 4,February 23, 2005Molecular Dream by Brian O'Hara. The 9 individual panels of this installation were painted with transparent glass paint on double layers of perspex and are based on coloured slides of common molecular biology themes. Brian O'Hara is a Dublin-based artist who lived in Germany for many years. The photograph was taken in the EMBO Building in Heidelberg on a sunny summer evening in 2004. Visit the artist at http://www.brian-ohara.com or send an e-mail to [email protected] Volume 24Issue 423 February 2005In this issue FiguresRelatedDetailsLoading ...