Abstract
BackgroundAge-dependent male osteoporosis remains a poorly studied medical problem despite its significance. It is estimated that at least 1 of 5 men will suffer from osteoporotic consequences. Given that multiple mechanisms are involved in the process of senescence, much attention has been given to compounds with polymodal actions. To challenge such a health problem, we tested here the therapeutic potential of resveratrol in male osteoporosis. We also studied the possible molecular mechanisms that may underlie resveratrol effects.MethodsThirty male Wistar albino rats were used in the present study. Rats were divided (10/group) into: control (3–4 months old weighing 150–200 g receiving vehicle), aged (18–20 months old, weighing 350–400 g and receiving vehicle), and resveratrol treated aged (18–20 months old, weighing 350–400 g and receiving resveratrol 20 mg/kg/day for 6 weeks) groups. Assessment of serum calcium, phosphate, bone specific alkaline phosphatase, inflammatory cytokines, oxidative stress markers, and rat femur gene expression of FoxO1, SIRT1, RANKL and OPG proteins was carried out. Histopathological assessment of different levels of rat femur was also performed.ResultsAge-dependent osteoporosis resulted in significant increase in serum levels of phosphate, bone specific alkaline phosphatase, hsCRP, IL-1β, IL-6, TNF-α, MDA, NO, and RANKL gene expression. However, there was significant decrease in serum level of GSH, and gene expression of FoxO1, SIRT1 and OPG. Osteoporotic changes were seen in femur epiphysis, metaphysis and diaphysis. Resveratrol restored significantly age-dependent osteoporotic changes.ConclusionWe concluded that resveratrol can play an important role in the prevention of male osteoporosis. Resveratrol can counter the molecular changes in male osteoporosis via anti-inflammatory, anti-oxidant and gene modifying effects.
Highlights
Age-dependent male osteoporosis remains a poorly studied medical problem despite its significance
Serum calcium level was significantly lower in resveratrol treated aged group when compared to the control group (11.75 ± 0.59 mg/dl), while it remained insignificantly different when compared to the aged rats (Fig. 1a)
Serum phosphate level was significantly lower in resveratrol treated aged group when compared to the aged group (3.18 ± 0.22 mg/dl), but was insignificantly different when compared to the control rats (Fig. 1b)
Summary
Age-dependent male osteoporosis remains a poorly studied medical problem despite its significance. Given that multiple mechanisms are involved in the process of senescence, much attention has been given to compounds with polymodal actions. To challenge such a health problem, we tested here the therapeutic potential of resveratrol in male osteoporosis. Osteoporosis represents a major health and societal burden for both men and women, only a minority of men are screened for osteoporosis or treated for fracture prevention [2]. Osteoporotic fractures represent a major public health problem worldwide because of the associated morbidity, mortality and costs. Considered as a disease of aging women, osteoporosis is becoming an increasingly important male health problem with one in three fragility fractures after the age of 50 years occurring in men [5]. Greater frailty may partly explain the increased fracture-related morbidity and mortality in men [7]
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