Abstract
Our recent work uncovered novel roles for activated Gαi signaling in the regulation of neutrophil polarity and adhesion. GαiGTP-mediated enhancement of neutrophil polarization was dependent on inhibition of cAMP/PKA signaling, whereas reversal of Gβγ-stimulated adhesion was cAMP/PKA independent. To uncover the mechanism for Gαi regulation of adhesion, we analyzed the effects of constitutively active Gαi1(Q204L) expression on adhesion driven by constitutively active Rap1a(G12V) or its downstream effector Radil in neutrophil-like HL-60 cells, or in HT-1080 fibrosarcoma cells. In HT-1080 cells, Rap1a(G12V) or Radil cause an increase in cell spreading and adhesion to fibronectin, which are both reversed by Gαi1(Q204L) but not WT Gαi1 In contrast, Gαi1(Q204L) did not reverse Rap1-GTP-interacting adaptor molecule (RIAM)-dependent increases in cell adhesion. This indicates that adhesion regulation by Gαi-GTP occurs downstream of Rap1a and Radil, but is upstream of components such as integrins and talin that are regulated by both Radil and RIAM. HL-60 neutrophil-like cells expressing Rap1a(G12V) or Radil have an elongated phenotype because of enhanced uropod adhesion as they attempt to migrate on fibronectin. This elongated phenotype driven by Rap1a(G12V) or Radil is reversed by Gαi1(Q204L), but not by WT Gαi1 expression, suggesting that Gαi-GTP also regulates adhesion in immune cells at the level of, or downstream of, Radil. These data identify a novel role of Gαi-GTP in regulation of cell adhesion and migration. Cell migration involves cycles of adhesion and de-adhesion, and we propose that the dynamic spatiotemporal balance between Gβγ-promoted adhesion and Gαi-GTP reversal of adhesion is important for this process.
Highlights
Our recent work uncovered novel roles for activated G␣i signaling in the regulation of neutrophil polarity and adhesion
To uncover the mechanism for G␣i regulation of adhesion, we analyzed the effects of constitutively active G␣i1(Q204L) expression on adhesion driven by constitutively active Rap1a(G12V) or its downstream effector Radil in neutrophil-like HL-60 cells, or in HT-1080 fibrosarcoma cells
G␣i-GTP inhibits cell spreading and adhesion Neutrophils and neutrophil-like cell lines where G␥-dependent integrin regulation is critical for cell adhesion and migration are difficult to manipulate genetically
Summary
G␣i-GTP inhibits cell spreading and adhesion Neutrophils and neutrophil-like cell lines where G␥-dependent integrin regulation is critical for cell adhesion and migration are difficult to manipulate genetically. In HT-1080 fibrosarcoma cells an fMLP–Rap1a–Radil–1 integrin pathway analogous to that operating in neutrophils regulates adhesion to fibronectin, providing a tractable model to dissect G␣i effects
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