Abstract
We describe malignant transformation of cultured rat intestinal epithelial cells (IEC-18) by transfection with the activated human H- ras gene cloned from the EJ bladder carcinoma. Transformed cells showed a marked morphological change, expressed high levels of the transfected H- ras gene, were able to grow in agar and expressed antigenic markers identical with parental IEC-18 cells. When injected into syngeneic rats these cells formed rapidly growing tumors expressing the same intestinal-specific antigenic markers as the injected cells. Parallel to the high expression of H- ras mRNA in the transformants we document overexpression of rat α-TGF mRNA.
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