Abstract
In vitro oxidation is a problem for some herbaceous and woody species and can cause darkening of tissues and consequently death of explants and plants Therefore, this study aimed to assess the effect of activated charcoal on in vitro yam cultivation, aiming at reducing or eliminating explant oxidation and optimizing the growth of the genotypes Dioscorea alata var. purpurea (Roxb.) A. Pouchet and Dioscorea rotundata Poir. Nodal segments of approximately 1 cm, extracted from plants previously grown in vitro, were introduced into test tubes containing 10 mL of 2GGC culture medium, plus 30 g L-1 sucrose, solidified with 2.2 g L-1 Phytagel® and pH adjusted to 5.8 before autoclaving, containing activated charcoal doses of 0, 1, 2, 3 and 4 g L-1. Plants were maintained for 90 days in a growth room, with temperature of 27 ± 1ºC, photon flux density of 30 μmol m-2 s-1 and photoperiod of 16 hours, after which their development variables were evaluated. Activated charcoal, at the concentration of 4 g L-1 considerably promoted the best development of plants, and the species D. alata var. purpurea showed higher means for all variables studied.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.