Abstract

The tachykinin-dependent stimulation of ion transport across frog skin was studied. Tachykinin stimulation was due to interaction with an NK1-like receptor as [Sar 9-Met(O 2) 11]-Substance P (a very selective NK1 agonist) strongly stimulated SCC, whereas [β-Ala 8]-Neurokinin A 4–10 (a very selective NK2 agonist) did not. The rank order of tachykinin potency was: PG-KI > Uperolein > Hylambatin > Kassinin > Phyllomedusin > [Sar 9-Met (O 2) 11]-Substance P > Ranatachykinin A > Physalaemin > Ranakinin > Substance P and Eledoisin ≫ Neurokinin A. Neurokinin B, Scyliorhinin I, Urechistachykinin I and Urechistachykinin II had no effect. We conclude that the minimal structural requirements for stimulating SCC in the frog skin were the presence of: a) the C-terminal sequence Phe-X-Gly-Leu-Met-NH 2; b) at least one Pro residue in the N-terminal sequence.

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