Actions of epinephrine on the contractility of fast and slow skeletal muscle fibres in teleosts

Abstract

Fast and slow muscle fibers were isolated from the myotomes of atlantic cod (Gadus morhua L.) and sculpin (Myoxocephalus scorpius L.). Epinephrine was found to have no effect on twitch or sub-tetanic contractions in fast muscle fibres. Isoprenaline (10(-6)M) had no effect on the contractility of slow muscle fibres. In contrast, epinephrine elicited a dose-dependent decrease in the half-time for twitch relaxation (t1/2r), and in most cases a decrease in twitch amplitude. The maximum decrease in t1/2r was around 5-20% of control values (at 10(-6)M epinephrine), with a half maximal response at about 30 nmol l(-1). Responses to epinephrine were unaffected by propranolol and reversed by phentolamine, consistent with the stimulation of α-adrenoreceptors. 10(-6)M epinephrine produced a rise in cAMP levels from 1.8 to 3.1 pmol mg dry wt(-1) in cod slow fibres. However, the cellular mechanism underlying the action of epinephrine is unclear since forskolin, a potent activator of adenylate cyclase activity, where it has been investigated, was found to increase not decrease twitch duration and amplitude. The responses of fast and slow fibres to epinephrine and its antagonists were similar in summer (13°C) and winter acclimatized (5-6°C) sculpin.It is suggested that epinephrine may act to modulate the active state of slow muscle fibres at high cruising speeds and thereby increase swimming performance.