Actions of amiodarone on mitochondrial ATPase and lactate dehydrogenase activities in guinea pig heart preparations

Abstract

The effects of amiodarone on mitochondrial ATPase (EC 3.6.1.3) and lactate dehydrogenase (LDH: EC 1.1.1.27) activities were studied in guinea pig mitochondrial preparations in order to test the hypothesis that amiodarone exerts some of its effects as a result of multiple actions on membrane-bound enzymes and receptors. Amiodarone inhibited the ATPase activity in the range of 10 pM to 10 mM (n = 10) with IC 50 values of 56.4±7.2 μM. However, although the inhibitory action was very significant (P<0.0001, compared to the control) in the concentration range of 100 pM to 10μM, the differences in individual enzyme responses showed very weak correlation with drug concentration. In this region, the inhibitory effects were almost constant at approximately 37%. Below 100 pM and above this range however, the concentration-response relationships were steep, reaching total inhibition at approximately 2.5 mM. Amiodarone also exerted concentration-dependent inhibitory effects on lactate dehydrogenase activity. However, over the effective inhibitory concentration range (5–95%) of 7.5 μM to 2.5 mM (n = 8) and IC 50 value of 108±6 μM, its inhibitory potency was two fold of its ATPase inhibition. We propose that these actions contribute, at least in part, to the mechanism(s) of some of the pharmacological actions of amiodarone.