Abstract
An action spectrum was obtained for the suppression of arylalkylamine N-acetyltransferase (NAT) activity in the two-spotted spider mite Tetranychus urticae by irradiating the mite with monochromatic lights of various wavelengths using the Okazaki Large Spectrograph at the National Institute for Basic Biology, Okazaki, Japan. Fluence-response curves were obtained for wavelengths between 300 and 650 nm by irradiating the mite for 4 h day(-1). The samples were frozen after the third exposure. A negative correlation between the logarithmic fluence rate and NAT activity was detected in the range of 0.01-1 micromol m(-2) s(-1) for wavelengths between 300 and 500 nm and in the range of 0.1-10 micromol m(-2) s(-1) for wavelengths between 550 and 650 nm. The constructed action spectrum indicated that the photoreceptors mediating the circadian and/or photoperiodic systems might be UV-A- and blue-type photoreceptors with absorption peaks at 350 and 450 nm.
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