Action potentials pass severed earthworm MGA segments reconnected by laser and electric field pulses

Abstract

Active and passive electrical signals transmit across the earthworm ( Lumbricus terrestris) medial giant axon (MGA) segment after reconnection induced by laser and electric field pulses. Laser pulses of 308 nm, 15 ns pulse duration, and pulse energy 5–50 μJ in the objective plane induced axon reconnection in 12 of 133 (9%) preparations. Electric field pulses of 200–800 V,100 μs duration, induced axon reconnection in 15 of 188 (8%) preparations. Electrical continuity is possible after the reconnection of severed axons induced by laser and electric field pulses.