Abstract
Cyclomalto-oligosaccharides ( α-, β-, and γ-cyclodextrins) were partially oxidized by sodium meta-periodate, yielding the dialdehyde forms (D-CDs). Reduction of the D-CDs by sodium borohydride gave the corresponding alcohols (D-CD/Rs). Column chromatography on Sephadex G-15 was effective in separating cyclomalto-hexaose and -heptaose ( α- and β-CD) derivatives from native CD. The susceptibility of α- and β-CD to Taka-amylase A increased markedly on conversion into derivatives, as judged by the decrease in the K m values. D-CDs were better substrates for cyclodextrin glucanotransferase than the D-CD/Rs and native CDs when the reaction assayed was hydrolysis of the CD. The observed increases in susceptibility to enzymes could be ascribed to a loosening of the cyclic structure of the substrates by the oxidative cleavage of carbon-carbon bonds.
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