Abstract
The effect of rubratoxin B on the electron transport system of mouse liver mitochondira was investigated. Oxygen consumption in mitochondira isolated from male mice was measured polarographically in a Gilson Oxygraph Model K-ICC. Oxygen consumption was depressed 73% at a concentration of 1.13 mM rubratoxin. At concentrations as low as 0.08 mM rubratoxin B, 50% inhibition was observed. Rubratoxin B (0.28 mM) depressed oxygen consumption 67% in ADP-coupled mitochondria and 60% in 2,4-dinitrophenol (DNP)-uncoupled mitochondira using either pyridine-nucleotide linked substrates or succinate. By employing the N, N, N′, N′-tetramethyl- p-phenylenediamine (TMPD) shunt, it was shown that inhibition was not between cytochrome b and cytochrome C 1 or C. It appears, based on these studies and comparison with known inhibitors of specific sites along the electron transport system that the principal site of action of rubratoxin B is between cytochrome C 1 or C and the termination of electron flow.
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