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Abstract
Using a thermal activity monitor (TAM) air isothermal microcalorimeter with ampoule mode, the thermo-genic curves of the metabolism of Tetrahymena thermophila BF 5 growth at 28 °C were obtained and the action of palmatine on it was investigated. Meanwhile, the biomass change during the process of T. thermophila BF 5 growth coexisted with palmatine was studied by a haemacytometer. The results showed that a low concentration (50 μg/mL) of palmatine began to inhibit the growth of T. thermophila BF 5, and when the concentration of palmatine reached 600 μg/mL, T. thermophila BF 5 could not grow at all. The relationship between the growth rate constant ( k) and the concentration c was almost linear with the correlation coefficient of 0.9957, showing the strong toxic action of palmatine on T. thermophila BF 5 growth. The biomass during T. thermophila BF 5 growth decreased obviously by the addition of palmatine at different concentrations. The investigation of biomass agreed well with the results obtained by means of microcalorimetry.
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