ACTION OF ISO-IMMUNE ANTI-M AND ANTI-L ON ACTIVE POTASSIUM TRANSPORT IN HK AND LK SHEEP RED CELLS

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This chapter discusses the action of isoimmune anti-M and anti-L on active potassium transport in high potassium (HK) and (LK) sheep red cells. Active and passive transport of sodium (Na) and potassium (K) are different in HK and LK sheep. Active transport of these ions and the Na+- K-stimulated and ouabain sensitive ATPase activity are about four times greater in HK red cells as compared to LK red cells. These cell types are also different with respect to their antigenic properties. Red cells of all HK sheep possess the M antigen whereas red cells of some LK sheep do not possess the M antigen. LK cells contain the L-antigen, which is absent in HK red cells. Anti-M serum has no effect on K-transport in HK and LK sheep red cells. Anti-M lyses HK red cells in the presence of guinea pig complement. HK red cells and their membrane bound this antibody with a high affinity while homozygous LK cells do not absorb anti-M.