Action of glucagon and glucagon-like peptide on glycogen metabolism of trout isolated hepatocytes

Abstract

1. 1. The incubation of isolated trout hepatocytes in the presence of salmon glucagon, mammalian glucagon, salmon glucagon-like peptide (GLP) induced an increase in the content of cyclic adenosine 3′,5′-monophosphate (cAMP), in the glycogen phosphorylase a activity, in the glucose release, and a decrease in the glycogen level in these cells. 2. 2. GLP showed a greater potency than either glucagon, and the same activity as equimolar doses of epinephrine. 3. 3. Results differ from those obtained in catfish hepatocytes, in which GLP had a very low activity compared to glucagon and epinephrine.