Abstract
Abstract The action of β-glucosidase on the methyl derivatives of p-nitrophenyl β-d-glucopyranoside (pNP-β-Glc), which were regio-specifically substituted at O-2, O-3, O-4, and O-6 positions, was studied. Specifically, several β-glucosidases isolated from brown-rot, white-rot, soft-rot fungi, and almond were investigated. These β-glucosidases did not act on the 2, 3, and 4-O-methyl derivatives, while the 6-O-methyl one was hydrolyzed by all the enzymes to some extent. The results indicate that the methyl group at O-2, O-3, and O-4 of the glucopyranoside strongly inhibits the recognition by the β-glucopyranoside, while the enzymes do not discriminate the structure difference between pNP-β-Glc and its methyl derivative at O-6.
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