Abstract
We examined the effect of ampicillin and kanamycin on the electrophysical characteristics of ampicillin- and kanamycin-sensitive and ampicillin- and kanamycin-resistant Escherichia coli cells. Substantial changes in the orientational spectra (OS) of suspensions of cells incubated with various ampicillin and kanamycin concentrations took place only at the first five frequencies of the orienting electric field (10–1000 kHz). The maximal change in the magnitude of the electro-optical signal occurred at 50 µg mL−1 of ampicillin and 10 µg mL−1 of kanamycin. The suspension-OS changes did not depend on the antibiotic-action period. Under the action of ampicillin and kanamycin, sensitive and resistant E. coli strains gave different electro-optical (EO) effects. It follows that the sensitive and resistant E. coli strains exhibit different of the EO effect on the action of ampicillin and kanamycin. Thus, the suspension-OS changes occurring under the effect of ampicillin and kanamycin may be used as a test for resistance to this antibiotic in the cells studied.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Environmental Analytical Chemistry
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
