Action of 1-β- d-Arabinofuranosylcytosine on mammalian tumor cells—2 : Inhibition of mammalian and oncogenic viral polymerases

Abstract

1. (1) 1-β- d-Arabinofuranosylcytosinetriphosphate (ara-CTP) is an inhibitor of: DNA-dependent DNA polymerase from mammalian cells, RNA-dependent DNA and DNA-dependent DNA polymerase from Rauscher murine leukemia virus (RMLV) and RNA-dependent DNA polymerase from mouse lymphoma cells. 2. (2) All these inhibitions are of the competitive type. The RNA-dependent DNA polymerases from oncogenic RNA viruses and from lymphoma cells are found to be most sensitive. These enzymes are approximately 200 times as sensitive to ara-CTP as DNA-dependent DNA polymerases.