Action de l'hormone juvenile et de certains mimetiques sur l'epithelium intestinal chez Galleria mellonella

Abstract

We have investigated the influence of juvenile hormone (JH) on the intestinal epithelium of G. mellonella, in vivo and in vitro. The larvae undergoing a supernumerary instar present a typical larval epithelium with columnar (CL) and globlet (CF) cells; the spinning period is characterized by a delay and a loss of synchronism in the process of differentiation of intermediates cells (Ci) typical of the pharate pupa. The larval-pupal intermediates show true mosaïcs in which Ci and CF are juxtaposed; however, the ratio of Ci in the epithelium progressively increases. The injection of JH at the beginning of spinning induces the appearance of CF just as Ci should normally grow. Hormone administration during the second half of the spinning period modifies the differentiation of epithelial cells: they become taller. We consider them to be cells engaged in pharate pupal differentiation, and which have then been partially oriented toward larval differentiation. These results show that the intestinal epithelium is a competent tissue, the sensibility of which to JH, is higher than that of the epidermis. The basal cell plasticity is very important and the action of JH on their differentiation may lead to CL or CF, to tall cells, and to Ci, depending on hormonal rate. In vitro, the experiments show that the action of JH is probably direct on the target tissue. The fact that JH can act very late as a modifier of the differentiation of the growing epithelial cells exclude the possibility that the hormone exercises its control through DNA replication.