Actinomycin Synthesis in Washed Cells of Streptomyces antibioticus

Herbert Weissbach,Betty G Redfield,Vida Beaven,Edward Katz

doi:10.1016/s0021-9258(18)97072-9

Abstract

Actinomycin Synthesis in Washed Cells of Streptomyces antibioticus

Highlights

Actinomycin Synthesis by Washed Mycelium-It was observed that washed mycelium of X. antibioticus, suspended in phosphate buffer, pH 7.4, incorporated amino acids into actinomycin at about two-thirds the rate seen with mycelium suspended in the glutamate-galactose medium
Actinomycin synthesis has been studied with 14C-labeled precursors in washed mycelium of Xtreptomyces antibioticus
Stimulation of X-amino acid incorporation into actinomycin is achieved upon the addition of the remaining amino acid precursors of the antibiotic or with the addition of 3-hydroxyanthranilic acid, 3-hydroxykynurenine, and 4-methyl-3-hydroxyanthranilic acid

Summary

Introduction

With the use of washed cells of Streptomyces antibioticus, it has been possible for the first time to stimulate antibiotic synthesis with both amino acids and tryptophan metabolites. E$ect of various amino acids on stimulation incorporation into actinomycin of 14C-valine The absence of tryptophan abolished the amino acid stimulation whereas the omission of either proline or methionine had only a slight effect.

Results

Conclusion