Abstract
Actinomycin D, when added to differentiating Dictyostelium discoideum cells, completely inhibits synthesis of ribosomal RNA but only partially inhibits synthesis of mRNA. Hence, earlier experiments using this drug, which indicated that the period of synthesis of mRNAs for several developmentally regulated enzymes preceded the period of translation by several hours, must be re-evaluated. We show here that a combination of actinomycin D and daunomycin does rapidly inhibit synthesis of mRNA; also there is a rapid and complete inhibition of biosynthesis of the three developmentally regulated enzymes studied. One interpretation of these results is that translation of mRNA follows closely upon synthesis of the mRNA, and that the primary control of protein biosynthesis during Dictyostelium development is at the level of mRNA transcription.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
