Actinomycete strain type determines the monodispersity and antibacterial properties of biogenically synthesized silver nanoparticles

Mostafa Mabrouk,Tarek A Elkhooly,Shaimaa K Amer

doi:10.1186/s43141-021-00153-y

Abstract

BackgroundBio-nanotechnology is considered as one of the low-cost approaches that have been utilized in production of nanomaterials. The current research aimed at investigating the influence of different types of Actinomycete strains on the final properties of silver nanoparticles (AgNPs) such as size, shape, polydispersity, and antibacterial properties. For this purpose, the following techniques were employed UV spectrophotometer, SDS-PAGE electrophoresis, TEM, FTIR, antibacterial agar diffusion test, and Zetasizer. ResultsIt was found that among 34 Streptomyces isolates collected from the soil, Streptomyces spiralis and Streptomyces rochei were able to reduce silver nitrate into sliver nanoparticles. The diversity and molecular weights of extracellular proteins secreted by these stains were different as proved by SDS-PAGE technique. This consequently resulted in differences in polydispersity of AgNPs which indicate that the sizes of AgNPs were highly dependent on the amount, molecular sizes, and diversity of extracellular matrix proteins of the microorganism. ConclusionThis article might give an insight about the importance of molecular sizes of biomacromolecules such as proteins on the physical properties of biogenic synthesized nanoparticles.

Highlights

Bio-nanotechnology is considered as one of the low-cost approaches that have been utilized in production of nanomaterials
Actinobacteria isolation and biosynthesis of arrangement of silver nanoparticles (AgNPs) The current study was focused on the extracellular synthesis of AgNPs from actinobacteria supernatant
A total of 34 actinomycetes isolates were isolated from collected soil sample from Halayeb and Shalatene area checked for their ability to produce AgNPs

Summary

Introduction

Bio-nanotechnology is considered as one of the low-cost approaches that have been utilized in production of nanomaterials. The current research aimed at investigating the influence of different types of Actinomycete strains on the final properties of silver nanoparticles (AgNPs) such as size, shape, polydispersity, and antibacterial properties. For this purpose, the following techniques were employed UV spectrophotometer, SDSPAGE electrophoresis, TEM, FTIR, antibacterial agar diffusion test, and Zetasizer. Actinobacteria are gram-positive filamentous bacteria which are widely found in both terrestrial and marine environment and have long been exploited commercially as an amusing source of unique secondary metabolites, such as antibiotics, enzymes, and enzyme inhibitors [16, 17] They were recognized as an efficient possibility for metal nanoparticles synthesis creation extracellularly and intracellularly. They have demonstrated significant biocidal activity against various microorganisms [18, 19]

Methods

Results

Discussion

Conclusion